Bacterial flora-typing with targeted, chip-based Pyrosequencing

Lyssavirus Detection and Typing Using Pyrosequencing 1

Lyssavirus detection and typing using pyrosequencing.

Rabies is a fatal zoonosis caused by a nonsegmented negative-strand RNA virus, namely, rabies virus (RABV). Apart from RABV, at least 10 additional species are known as rabies-related lyssaviruses (RRVs), and some of them are responsible for occasional spillovers into humans. More lyssaviruses have also been detected recently in different bat ecosystems, thanks to the application of molecular d...

Array-based binary analysis for bacterial typing.

An allele-specific oligonucleotide microarray was developed for rapid typing of pathogens based on analysis of genomic variations. Using a panel of Escherichia coli strains as a model system, selected loci were sequenced to uncover differences, such as single- or multiple-nucleotide polymorphisms as well as insertion/deletions (indels). While typical genomic profiling experiments employ specifi...

Hand wound with unusual bacterial flora.

A young soldier was admitted to hospital late one night with it deep wound of the dorsum of his right hand, caused, he said, by a fall. (On subsequent questioning, the patient revealed that he had been involved in a fight, during the course of which he had struck his opponent in the mouth and received a bite.) The wound appeared clean at the time and was· therefore primarily sutured. The patien...

Molecular Methods for Bacterial Strain Typing

ABSTRACT        Typing of bacteria is an important part of epidemiological studies on nosocomial infections. Bacterial identification methods have dramatically improved in recent years, which is mainly due to advancements in the field of molecular biotechnology. In many cases, molecular techniques have replaced phenotypic typing methods. Currently, a wide r...